However,its role in trophectoderm(TE) remains unknown.METHODS:In the present study,we investigat…
【目的】PM2.5对机体多个组织和细胞都有危害作用,星形胶质细胞的激活发生在各种中枢神经系统损伤的反应中起着重要作用。在本研究中,我们探讨PM2.5能否诱导星形胶质细胞的炎症活化以及其机制。【材料和方法】采用C6星形胶质细胞观察PM2.5诱导星形胶质细胞炎症激活以及通路。首先,采用Western
blot、酶联免疫吸附试验方法和半定量RT-PCR方法检测PM诱导星形细胞C6炎症因子iNOS、TNF-α和IL-1β的表达和分泌;其次,采用Western blot方法检测PM2,。5引起的C6细胞的总蛋白中STAT3、p-STAT3、JAK2和P-JAK2的蛋白水平变化,为进一步确证JAK2/ST…
Objective To investigate the effect of p38 MAPK on the expression of MRP1 and drug resistance with refractory epileptic rats.Methods We used a rat model of refractory epilepsy to examine whether p38 MAPK affect the expression of MRP1 and the concentrations of AEDs in the brain.The expression of M…
目的多药转运体P-糖蛋白(P-glycoprotein,PGP)介导的难治性癫痫耐药己被国内外公认。但PGP表达上调的分子机制目前尚未清楚。我们前期研究发现在体外培养的K562细胞上,p38 MAPK信号通路调控了PGP的表达。但在难治性癫痫大鼠脑内p38 MAPK信号通路是否调控了PGP的表达,并参与了癫痫的耐药目前尚不清楚。本实验旨在探索在难治性癫痫大鼠脑内p38 或者 MAPK信号通
目的观察p38 MAPK对难治性癫痫大鼠脑内MRP1表达的影响及对癫痫大鼠海马细胞外液抗癫痫药物浓度的影响,从而明确p38 MAPK是否参与了难治性癫痫的耐药。方法健康雄性SD大鼠,随机分成两组,分别是正常对照组(n=16)和模型组(n=44),采用戊四氮腹腔注射
Circulating angiotensinⅡ(AngⅡ) levels are elevated in hypertensive patients,while cyclooxygenase -2(COX-2) may act as the culprit selleck of vascular inflammation
and is found in atherosclerotic plaques.Although both AngⅡand COX-2 are associated with vascular inflammation and remodeling,it remains elusiv…
缺血及再灌注(Ischemia and reperfusion,I/R)是心脑血管疾病及治疗过程中常见的病理改变。远距预处理(Remote preconditioning,remote PC)则是种通过短暂的远离靶器官的远端组织的缺血起到靶器官保护作用的技术。1993年Przyklenk等发现,对左旋支施行预处理可明显增强前降支所支配区域心肌的
Objective Our previous study has demonstrated
that in response to hypoxia and 以及 serum deprivation(hypoxia/SD),both components of ischemia in vivo,rat bone marrow-derived mesenchymal stem cells(MSCs)underwent the caspase-dependent apoptosis involving changes in mitochondrial integrity and function.
AIMS: To investigate possible relationship of caveolin-1 and SR-A in foam cells derivedfrom macrophage induced by ox-LDL and explore possible molecular mechanisms.METHORDS: RAW 264.7 mouse macrophages were used. Western blot analysis was usedto detect protein expression. High performance liquid …
目的观察。肾上腺髓质素(adrenomedullin,ADM)在人喉癌中表达、分布,探讨其临床意义及相关机制。方法运用半定量RT-PCR 方法检测喉癌组织、非癌喉粘膜中肾上腺髓质素mRNA 的表达差异;运用免疫组化方法观察喉癌标本中肾上腺髓质素分布规律;运用放射免疫测定人喉鳞癌细胞系hep-2培养基、不同组织标本、血浆中ADM 浓度:运用氚胸腺嘧啶(H~3-TDR)掺入方法观察肾上腺髓质素促进hep-2细胞增殖效应以及信号传导通路。结果H~3-PCR 结果发现在hep-2细胞、喉癌组织、非癌喉黏膜中均可以看到ADM 基因扩增。喉癌组织ADM 基因表达水平较非癌喉黏膜高2.96倍,(F=73.2…
Attachment of leukocytes to the vascular endothelium and the subsequent migration of cells into the vessel wall are early events in atherogenesis. Expression of endothelial adhesion molecules play an important role in this process. In the present study, we tested the effect of Epigllocatechin-3-…