在乳腺癌中,上皮间质转化是肿瘤转移的必须步骤。现有的抗肿瘤转移药物的作用机理大都是杀伤肿瘤细胞来减少肿瘤数目。这种杀伤作用对正常细胞也造成了伤害,给病人带来了巨大的痛苦。 在这项研究中,我们用4T1细胞系建立了Balb/c小鼠的乳腺癌自发转移模型。在这个模型中,我们发现,五味子乙素可以减少乳腺癌的肺转移灶数目,延长乳腺癌荷瘤小鼠的生存时{Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|selleck Anti-infection Compound Library|selleck Antiinfection Compound Library|selleck Anti-infection Compound Library|selleck Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library半抑制浓度|Anti-infection Compound Library价格|Anti-infection Compound Library花费|Anti-infection Compound Library溶解度|Anti-infection Compound Library购买|Anti-infection Compound Library制造商|Anti-infection Compound Library查找购买|Anti-infection Compound Library订单|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library分子量|Anti-infection Compound Library molecular weight|Anti-infection Compound Library数据表|Anti-infection Compound Library supplier|Anti-infection Compound Library体外|Anti-infection Compound Library细胞系|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library体内|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library半抑制浓度|Antiinfection Compound Library价格|Antiinfection Compound Library花费|Antiinfection Compound Library溶解度|Antiinfection Compound Library购买|Antiinfection Compound Library制造商|Antiinfection Compound Library查找购买|Antiinfection Compound Library订单|Antiinfection Compound Library chemical structure|Antiinfection Compound Library数据表|Antiinfection Compound Library supplier|Antiinfection Compound Library体外|Antiinfection Compound Library细胞系|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening|间。对早期、中期和晚期乳腺癌的手术治疗的同时辅以五味子乙素,均可以使小鼠生存时间延长。病理切片提示,五味子乙素降低了肿瘤细胞对周围肌肉组织的侵袭,使肿瘤细胞之间保持相对紧密的连接,抑制了其向间质细胞形态的转化。免疫组化的结果证明,所有这些现象的原因在于五味子乙素抑制了体内乳腺癌细胞的上皮间质转化过程。 细胞实验Selleckchem Sorafenib证明,五味子乙素抑制了TGF-β引起4T1细胞的形态变化。划痕试验证明,五味子乙素可以降低肿瘤细胞的迁移能力。Transwell实验证明,五味子乙素降低了肿瘤细胞的侵袭能力。免疫荧光实验及Western蛋白电泳实验证明,五味子乙素抑制了4T1细胞的上皮间质转化过程。 综上所述,本项研究证明,五味子乙素在体内、体PFTα 价格外均可以抑制乳腺癌细胞的上皮间质转化。五味子乙素具有较低的毒性,对正常细胞及肿瘤细胞均没有杀伤作用。目前,临床上尚无此类只作用于肿瘤转移过程的药物。本实验的结果表明,五味子乙素具有巨大的临床应用价值和开发前景。本课题的研究目的在于为五味子乙素临床应用提供理论依据和基础数据。
目的： 本文旨在探索促红细胞生成素（EPO）调控心肌成纤维细胞toll样受体4（TLR4）表达的生物学效应和机制。